Involvement of Pseudomonas aeruginosa Rhodanese in Protection from Cyanide Toxicity
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چکیده
منابع مشابه
Involvement of Pseudomonas aeruginosa rhodanese in protection from cyanide toxicity.
Cyanide is a serious environmental pollutant and a biocontrol metabolite in plant growth-promoting Pseudomonas species. Here we report on the presence of multiple sulfurtransferases in the cyanogenic bacterium Pseudomonas aeruginosa PAO1 and investigate in detail RhdA, a thiosulfate:cyanide sulfurtransferase (rhodanese) which converts cyanide to less toxic thiocyanate. RhdA is a cytoplasmic enz...
متن کاملThe isolation of rhodanese from Pseudomonas aeruginosa by affinity chromatography.
Rhodanese (thiosulphate : cyanide sulphur-transferase; EC 2 . 8 . I . I) was originally ,described by Lang (1933). The enzyme catalyses the formation of thiocyanate from cyanide and thiosulphate according to the reaction : S20!+ CN-+ SCN+ SO:-. Rhodanese activity has been demonstrated in most mammalian tissues, with the greatest activity present in liver and kidney. The enzyme has been purified...
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I nfection and subsequent chronic infection of the cystic fibrosis (CF) lung with organisms such as Pseudomonas aeruginosa probably occurs at an early stage in the life of a child suffering from CF. Clinicians worldwide are eager to isolate the organism as early as possible in an attempt to treat the patient before any permanent damage occurs and hopefully to eliminate the organism completely f...
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technique, and 9 of 20 when an anaerobic jar was used). Thus, anaerobic fermentation of mannitol seems to be too exacting a criterion, but all methods using glucose fermentation classified the 20 coagulase-positive strains as staphylococci. The classification of other staphylococci into S. saprophyticus and S. lactis on their ability to produce acetoin from glucose (Shaw et al.) was not support...
متن کاملCyanide production by Pseudomonas fluorescens and Pseudomonas aeruginosa.
Of 200 water isolates screened, five strains of Pseudomonas fluorescens and one strain of Pseudomonas aeruginosa were cyanogenic. Maximum cyanogenesis by two strains of P. fluorescens in a defined growth medium occurred at 25 to 30 degrees C over a pH range of 6.6 to 8.9. Cyanide production per cell was optimum at 300 mM phosphate. A linear relationship was observed between cyanogenesis and the...
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ژورنال
عنوان ژورنال: Applied and Environmental Microbiology
سال: 2007
ISSN: 0099-2240,1098-5336
DOI: 10.1128/aem.02143-06